Journal: Developmental Cell

Article Title: DNGR-1-tracing marks an ependymal cell subset with damage-responsive neural stem cell potential

doi: 10.1016/j.devcel.2022.07.012

Figure Lengend Snippet: DNGR-1-tracing marks a distinct class of ependymal cells, wherein resides latent stem cell ability (A) Spinal cord central canal cryosection from a Clec9a Cre/Cre Rosa LSLtdTomato/LSLtdTomato mouse labeled with anti-CD133 (green). CD133 is expressed apically and marks all ependymal cells (traced and untraced). (B) FACS-sorting of CD133 + DNGR-1-traced and CD133 + non-traced cells. (C) Neurosphere assay of CD133 + DNGR-1-traced and CD133 + non-traced cells from 4 pooled spinal cords. Right, quantification of neurosphere-containing wells and number of neurospheres per well from each fraction. Data are from two independent experiments. Error bars (SEM). Groups were compared using unpaired t test. ∗∗∗∗ p < 0.0001. (D) Dataset integration and UMAP of CD133 + DNGR-1-traced cells and CD133 + non-traced cells into 3 groups. (E) Heatmap of the top 20 differentially expressed genes in each group from (D). (F) UMAP representation by cellular provenance and quantification of each cellular fraction per group. Orange, CD133 + DNGR-1-traced cells; Green, CD133 + non-traced cells. 97.89% of CD133 + DNGR-1-traced cells were unbiasedly assigned to ependymal cell group (group 2). The remaining CD133 + DNGR-1-traced cells were assigned to groups 1 (1.89%) and 3 (0.23%). Dashed line represents the proportion of CD133 + DNGR-1-traced cells within the UMAP space (15.95%—4,825 of 30,326 cells). (G) UMAP of cells from group 2 (from F). (H) UMAP shown in (G) indicating cellular provenance: CD133 + DNGR-1-traced ependymal cells in salmon and CD133 + non-traced cells in cyan. (I) Percentage of traced and non-traced cells in each of the clusters 0–4 from (G). Dashed line represents the proportion of CD133 + DNGR-1-traced cells within UMAP space (67.9%—4,723 of 6,951 cells). (J) Expression of a selected set of cluster markers from clusters 1 and 3 (from G). (K) Distribution of the top 5 Gene Ontology biological processes defined by the differentially expressed genes in each cluster. Scale bars, 500 μm (C) and 10 μm (A).

Article Snippet: For comparison of the neurosphere-forming potential between CD133 + DNGR-1-traced and CD133 + non-traced cells, spinal cords from Clec9a Cre/Cre Rosa LSLtdTomato/LSLtdTomato mice were digested and myelin-depleted as above and stained with anti-CD133 AF488 (1:100, clone 134A, BD) and anti-CD45 AF647 (1:100, clone 30F-11, Biolegend).

Techniques: Labeling, Neurosphere Assay, Expressing

Journal: Cytotechnology

Article Title: Doxorubicin and 5-fluorouracil resistant hepatic cancer cells demonstrate stem-like properties

doi: 10.1007/s10616-012-9511-9

Figure Lengend Snippet: Immunophenotype and rhodamine 123 efflux of HCSCs. HCSCs weakly expressed CD13 (a), strongly expressed CD326 (b), CD133 (c), and CD90 (d). The HCSC candidate population highly exhibited rhodamine 123 efflux (h, i, j) compared to primary cells (e, f, g)

Article Snippet: Cells were then stained with anti-CD13-FITC, anti-CD90-PE and anti-CD133-PerCP; anti-CD326-PerCP monoclonal antibodies (BD Biosciences) for 30 min at 4 °C.

Techniques:

Journal: EBioMedicine

Article Title: Autophagy in endothelial cells regulates their haematopoiesis-supporting ability

doi: 10.1016/j.ebiom.2020.102677

Figure Lengend Snippet: Defective autophagy in BM ECs from PGF patients. (a) The EC phenotype was characterised by the positive expression of CD34, CD133 and CD309, and representative intracellular levels of (b) LC3, (c) Beclin-1 and (d) p62 in precultured BM ECs were analysed by flow cytometry. The intracellular levels of (e) LC3, (f) Beclin-1 and (g) p62 in precultured BM ECs from PGF patients, GGF patients and healthy donors were analysed by flow cytometry (MFI, mean±SEM). (h) Representative images (left panel) and quantification (right panel) of intracellular autophagosomes and autophagic vacuoles in cultivated BM ECs from PGF patients, GGF patients and healthy donors (original magnification, 10 × , scale bars represent 50 μm). (i) The CFU plating efficiency and (j) apoptosis rates of BM CD34 + cells after 7 days of coculture with BM ECs from PGF patients and GGF patients. Continuous variables were compared using the Mann–Whitney U test. All P -values<0.05 were considered significant and are provided in the figure. * P <0.05, ** P <0.005, *** P <0.0005.

Article Snippet: After 7 days of cultivation, BM ECs were treated with rapamycin (5 μM) [ , , ] or HCQ (10 mΜ) for 6 h. The cultured BM ECs were characterised by staining with monoclonal mouse antibodies targeting human CD34, CD133, and vascular endothelial growth factor receptor 2 (VEGFR2, CD309) (Becton Dickinson) and running the labelled cells through a BD LSRFortessa cell analyser (Becton Dickinson) [ , , , , , ].

Techniques: Expressing, Flow Cytometry, MANN-WHITNEY

Journal: Exploration of medicine

Article Title: The association between circulating CD34+CD133+ endothelial progenitor cells and reduced risk of Alzheimer’s disease in the Framingham Heart Study

doi: 10.37349/emed.2024.00216

Figure Lengend Snippet: Examination of the relationship between different circulating progenitor or mature endothelial cells and the risk of AD/dementia by using Cox proportional hazards regression models after adjusting for covariates

Article Snippet: Then, PBMCs were isolated via Ficoll (General Electric) gradient centrifugation, counted, incubated with FcR blocking antibodies (Miltenyi Biotec), labeled with anti-human KDR PE antibody (R&D Systems), anti-human CD34 FITC antibody (BD Biosciences), anti-human CD133 APC antibody (BD Biosciences).

Techniques:

Journal: Exploration of medicine

Article Title: The association between circulating CD34+CD133+ endothelial progenitor cells and reduced risk of Alzheimer’s disease in the Framingham Heart Study

doi: 10.37349/emed.2024.00216

Figure Lengend Snippet: Detailed examination of the relationship between CD34+CD133+ (log-transformed) and the risk of AD/dementia risk

Article Snippet: Then, PBMCs were isolated via Ficoll (General Electric) gradient centrifugation, counted, incubated with FcR blocking antibodies (Miltenyi Biotec), labeled with anti-human KDR PE antibody (R&D Systems), anti-human CD34 FITC antibody (BD Biosciences), anti-human CD133 APC antibody (BD Biosciences).

Techniques:

Journal: Exploration of medicine

Article Title: The association between circulating CD34+CD133+ endothelial progenitor cells and reduced risk of Alzheimer’s disease in the Framingham Heart Study

doi: 10.37349/emed.2024.00216

Figure Lengend Snippet: Characteristics of the study sample based on circulating CD34+CD133+ EPC quartiles

Article Snippet: Then, PBMCs were isolated via Ficoll (General Electric) gradient centrifugation, counted, incubated with FcR blocking antibodies (Miltenyi Biotec), labeled with anti-human KDR PE antibody (R&D Systems), anti-human CD34 FITC antibody (BD Biosciences), anti-human CD133 APC antibody (BD Biosciences).

Techniques:

Journal: Exploration of medicine

Article Title: The association between circulating CD34+CD133+ endothelial progenitor cells and reduced risk of Alzheimer’s disease in the Framingham Heart Study

doi: 10.37349/emed.2024.00216

Figure Lengend Snippet: Survival analyses of circulating CD34+CD133+ cells for AD risk. Circulating the proportions (%) of CD34+CD133+ EPCs were divided into four quartiles based on the cell numbers (Q1 = 0.002–0.021; Q2 = 0.021–0.033; Q3 = 0.033–0.050; and Q4 = 0.050–0.609). Kaplan-Meier survival analysis was conducted to evaluate the survival-free time before AD over 14 years of follow-up to examine the relationship between CD34+CD133+ quartiles and AD incidence for: (A) all of the participants; (B) and (C) stratified based on the genotypes, (B) kirre like nephrin family adhesion molecule 3 (KIRREL3) rs4144611 major homozygotes TT vs. GG + TG; (C) KIRREL3 rs580382 major homozygotes CC vs. TT + CT, and (D) exocyst complex component 6B (EXOC6B) rs61619102 major homozygotes CC vs. GG + GC. P values are shown for statistical significance. Q: quartile

Article Snippet: Then, PBMCs were isolated via Ficoll (General Electric) gradient centrifugation, counted, incubated with FcR blocking antibodies (Miltenyi Biotec), labeled with anti-human KDR PE antibody (R&D Systems), anti-human CD34 FITC antibody (BD Biosciences), anti-human CD133 APC antibody (BD Biosciences).

Techniques:

Journal: Exploration of medicine

Article Title: The association between circulating CD34+CD133+ endothelial progenitor cells and reduced risk of Alzheimer’s disease in the Framingham Heart Study

doi: 10.37349/emed.2024.00216

Figure Lengend Snippet: The relationship between circulating CD34+CD133+ cells and reduced AD risk in the presence of vascular diseases. The FHS participants were stratified into divided based on the CD34+CD133+ EPC proportion quartiles and further divided into those individuals with the absence or presence of different vascular diseases. In the absence of any peripheral and central vascular diseases (A, B), in the presence of CHD (C, D), HTN (E, F), stroke (G, H), silent infarcts (I, J), CMBs (K, L), and high WMHI (M, N), the numbers and percentages of incident AD (A, C, E, G, I, K, and M) and all-cause dementia (B, D, F, H, J, L, and N) across four CD34+CD133+ quartiles were examined by using Chi-squared test with indicated P values. Q: quartile

Article Snippet: Then, PBMCs were isolated via Ficoll (General Electric) gradient centrifugation, counted, incubated with FcR blocking antibodies (Miltenyi Biotec), labeled with anti-human KDR PE antibody (R&D Systems), anti-human CD34 FITC antibody (BD Biosciences), anti-human CD133 APC antibody (BD Biosciences).

Techniques:

Journal: Exploration of medicine

Article Title: The association between circulating CD34+CD133+ endothelial progenitor cells and reduced risk of Alzheimer’s disease in the Framingham Heart Study

doi: 10.37349/emed.2024.00216

Figure Lengend Snippet: Comparisons of brain volumes across circulating CD34+CD133+ EPC quartiles

Article Snippet: Then, PBMCs were isolated via Ficoll (General Electric) gradient centrifugation, counted, incubated with FcR blocking antibodies (Miltenyi Biotec), labeled with anti-human KDR PE antibody (R&D Systems), anti-human CD34 FITC antibody (BD Biosciences), anti-human CD133 APC antibody (BD Biosciences).

Techniques:

Journal: Exploration of medicine

Article Title: The association between circulating CD34+CD133+ endothelial progenitor cells and reduced risk of Alzheimer’s disease in the Framingham Heart Study

doi: 10.37349/emed.2024.00216

Figure Lengend Snippet: GWAS on the interactive effects of genetic factors and circulating CD34+CD133+ cells on AD risk. The FSH analytical pipeline was used to perform GWAS. The interaction between genome-wide SNP dosage and log-transformed proportions (%) of circulating CD34+CD133+ EPCs for AD risk was tested by using the GEEPACK model (logistic regression utilizing generalized estimating equations) adjusting for age, sex, years of education, and the first 10 PCs. As shown in the Manhattan plot, 8 SNPs showed significant interactions with CD34+CD133+ frequency on AD risk (A). Seven of the identified SNPs with a P value < 5.0 × 10 −8 originated from gene KIRREL3 on chromosome 11 (rs580382 and rs4144611 were selected for further analysis), and 1 of them originated from EXOC6B (chromosome 2, rs61619102). Only r 2 ≥ 0.1 and SNPs with MAF ≥ 10% were considered. Manhattan plot (A) was used for visualization to identify the genotypes of two genes ( KIRREL3 and EXOC6B ) that had interactive effects with CD34+CD133+ EPCs for AD risk. Gene structures, surrounding regions, and methylation sites of KIRREL3 (B) and EXOC6B (C) are presented by using LocusZoom

Article Snippet: Then, PBMCs were isolated via Ficoll (General Electric) gradient centrifugation, counted, incubated with FcR blocking antibodies (Miltenyi Biotec), labeled with anti-human KDR PE antibody (R&D Systems), anti-human CD34 FITC antibody (BD Biosciences), anti-human CD133 APC antibody (BD Biosciences).

Techniques: Genome Wide, Transformation Assay, Methylation

Journal: Exploration of medicine

Article Title: The association between circulating CD34+CD133+ endothelial progenitor cells and reduced risk of Alzheimer’s disease in the Framingham Heart Study

doi: 10.37349/emed.2024.00216

Figure Lengend Snippet: Interactions between SNPs of KIRREL3 or EXOC6B and CD34+CD133+ on AD risk in FHS

Article Snippet: Then, PBMCs were isolated via Ficoll (General Electric) gradient centrifugation, counted, incubated with FcR blocking antibodies (Miltenyi Biotec), labeled with anti-human KDR PE antibody (R&D Systems), anti-human CD34 FITC antibody (BD Biosciences), anti-human CD133 APC antibody (BD Biosciences).

Techniques:

Journal: Exploration of medicine

Article Title: The association between circulating CD34+CD133+ endothelial progenitor cells and reduced risk of Alzheimer’s disease in the Framingham Heart Study

doi: 10.37349/emed.2024.00216

Figure Lengend Snippet: The association between circulating CD34+CD133+ cells and the risk of AD in the context of genetic background

Article Snippet: Then, PBMCs were isolated via Ficoll (General Electric) gradient centrifugation, counted, incubated with FcR blocking antibodies (Miltenyi Biotec), labeled with anti-human KDR PE antibody (R&D Systems), anti-human CD34 FITC antibody (BD Biosciences), anti-human CD133 APC antibody (BD Biosciences).

Techniques: